Modification-specific proteomics: Strategies for characterization of post-translational modifications using enrichment techniques
نویسندگان
چکیده
منابع مشابه
Current strategies and findings in clinically relevant post-translational modification-specific proteomics
Mass spectrometry-based proteomics has considerably extended our knowledge about the occurrence and dynamics of protein post-translational modifications (PTMs). So far, quantitative proteomics has been mainly used to study PTM regulation in cell culture models, providing new insights into the role of aberrant PTM patterns in human disease. However, continuous technological and methodical develo...
متن کاملCharacterization of Post-Translational Modifications Using Counting Approaches
There is an increasing interest in the identification and characterization of protein posttranslational modifications (PTMs). The vast amount of information obtained within a typical differential proteomics makes the measure of PTMs challenging. We have begun to explore counting methods for differential analysis of PTM changes in proteomes. We have developed a software program, the Software Too...
متن کاملBayesian false discovery rates for post-translational modification proteomics
Tandem mass spectrometry-based proteomics enables high throughput analysis of post-translational modifications (PTMs) on proteins. In current researches of shotgun proteomics, peptides with various PTMs and those without PTMs are often identified together and an overall false discovery rate (FDR) is estimated. However, it is often the case that only a subset of identifications, e.g. those with ...
متن کاملProteomics studies of post-translational modifications in plants.
Post-translational modifications of proteins greatly increase protein complexity and dynamics, co-ordinating the intricate regulation of biological events. The global identification of post-translational modifications is a difficult task that is currently accelerated by advances in proteomics techniques. There has been significant development in sample preparation methods and mass spectrometry ...
متن کاملPost-Translational Modifications Mapping Protein Networks Disease Proteomics
Protein post-translational modifications (PTMs) allow the cell to regulate protein activity and play a crucial role in the response to changes in external conditions or internal states. Advances in mass spectrometry now enable proteome wide characterization of PTMs and have revealed a broad functional role for a range of different types of modifications. Here we review advances in the study of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2009
ISSN: 1615-9853,1615-9861
DOI: 10.1002/pmic.200900398